Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance |
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Authors: | Carlos Romero José M Bellés José L Vayá Ramón Serrano Francisco A Culiáñez-Macià |
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Institution: | (1) Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia C.S.I.C. Camino de Vera s/n, E-46022 Valencia, Spain, ES |
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Abstract: | The yeast trehalose-6-phosphate synthase gene (TPS1) was engineered under the control of the cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants.
Using Agrobacterium-mediated transfer, the gene was incorporated into the genomic DNA and constitutively expressed in Nicotiana tabacum␣L. plants. Trehalose was determined in the transformants, by anion-exchange chromatography coupled to pulsed amperometric
detection. The non-reducing disaccharide accumulated up to 0.17 mg per g fresh weight in leaf extracts of transgenic plants.
Trehalose-accumulating plants exhibited multiple phenotypic alterations, including stunted growth, lancet-shaped leaves, reduced
sucrose content and improved drought tolerance. These pleiotropic effects, and the fact that water loss from detached leaves
was not significantly affected by trehalose accumulation, suggest that synthesis of this sugar, rather than leading to an
osmoprotectant effect, had altered sugar metabolism and regulatory pathways affecting plant development and stress tolerance.
Received: 8 July 1996 / Accepted: 10 October 1996 |
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Keywords: | : Drought tolerance Nicotiana Osmotic stress Transgenic plants Trehalose |
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