| 实时荧光定量PCR法研究结直肠癌患者肠道拟杆菌属、梭杆菌属和梭菌属量的变化 |
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| 引用本文： | 郭世奎,包维民,龚昆梅,胡大春,邵剑春,陈弟,王昆华.实时荧光定量PCR法研究结直肠癌患者肠道拟杆菌属、梭杆菌属和梭菌属量的变化[J].中国微生态学杂志,2010,22(1):24-28. |
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| 作者姓名： | 郭世奎 包维民 龚昆梅 胡大春 邵剑春 陈弟 王昆华 |
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| 作者单位： | 1. 云南省第一人民医院,昆明医学院附属昆华医院,普外一科/云南省肠内肠外营养研究中心,云南,昆明,650032
2. 昆明市第一人民医院,检验科,云南,昆明,650011 |
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| 基金项目： | 云南省应用基础研究项目 |
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| 摘 要： | 目的通过研究结、直肠癌患者肠道拟杆菌属、梭杆菌属和梭菌属量的变化,揭示肠道相关菌群改变在大肠癌发病中的作用及意义。方法收集术前结、直肠癌患者粪便标本40例及正常对照标本40例,根据细菌的靶基因序列设计特异性引物。提取待测粪便标本细菌DNA,应用SYBR Green I实时荧光定量PCR测定不同细菌的数量。结果正常对照组与实验组粪便中细菌数量分别为拟杆菌属(8.76±0.77;9.85±0.88)、梭杆菌属(7.94±1.25;10.0±1.65)、梭菌属(3.54±0.70;6.56±0.68),拟杆菌属中的脆弱拟杆菌为(2.12±0.48;4.07±1.77)、梭杆菌属中的坏死梭杆菌为(2.31±0.26;7.62±2.68)及梭菌属中的肉毒梭菌为(2.76±1.16;5.43±1.21),实验组数量均明显增多(P0.05)。结论结、直肠癌患者粪便中拟杆菌属、梭杆菌属和梭菌属的数量较正常对照明显增多,提示结、直肠癌的发生发展与肠道菌群有明显关系。
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| 关 键 词： | 结直肠癌 拟杆菌属 梭杆菌属 梭菌属 实时荧光定量PCR |
Real-time fluorescence quantitative-PCR analysis of the variation of Bacteroides,Fusobacterium and Clostridium in patients with colorectal cancer |
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| GUO Shi-kui,BAO Wei-min,GONG Kun-mei,HU Da-chun,SHAO Jian-chun,CHEN di,WANG Kun-hua.Real-time fluorescence quantitative-PCR analysis of the variation of Bacteroides,Fusobacterium and Clostridium in patients with colorectal cancer[J].Chinese Journal of Microecology,2010,22(1):24-28. |
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| Authors: | GUO Shi-kui BAO Wei-min GONG Kun-mei HU Da-chun SHAO Jian-chun CHEN di WANG Kun-hua |
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| Institution: | 1. Department of General Surgery, the First People＇s Hospital of Yunnan Province ( Kunhua Hospital Affliated to Kunming Medical College)/Parenteral and Enteral Nutrition Research center of Yunnan Province, Kunrrdng 550032, China ;2. Depart- ment of Clinical Laboratory, the First People＇s Hospital of Kunming , Kunming 650011, China) |
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| Abstract: | Objective To analyze changes in the mumber of Bacteroides,Fusobacterium and Clostridium in colorectal cancer patients by real-time fluorescence quantitative PCR and reveal the role and significance of gut microflora in colorectal cancer-associated molecular pathogenesis.Method The feces of preoperative colorectal cancer patients(n=40) and healthy volunteers(n=40) were collected.Specif primers were designed according to the target gene sequences of Bacteroides,Fusobacterium and Clostridium.Bacterial genome D... |
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| Keywords: | Colorectal cancer Bacteroides Fusobacteri- um Clostridium Real-time fluorescence quantitative PCR |
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