| 可溶性CD14基因克隆及序列分析 |
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| 引用本文： | 白洁,王威,荫俊,王占东,王忠泽.可溶性CD14基因克隆及序列分析[J].生物技术通讯,2001,12(2):94-95,98. |
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| 作者姓名： | 白洁 王威 荫俊 王占东 王忠泽 |
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| 作者单位： | 1. 军事医学科学院微生物流行病研究所
2. 白求恩医科大学 |
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| 摘 要： | 针对人可溶性CD14(sCD14）的cDNA序列设计引物，通过反转录PCR技术，从U937细胞中，扩增出编码人可溶性CD14的基因序列，并将其克隆至质粒pGEM－T中，通过PCR、酶切及序列测定鉴定重组载体。结果表明获得了人可溶性CD14基因片段，为下一步进行CD14表达及生物学活性研究奠定了基础。
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| 关 键 词： | CD14 基因克隆 序列分析 |
Cloning and sequencing of human soluble CD14 |
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| BAI Jie,WANG Wei,YIN Jun,WANG Zhan-dong,WNAG Zhong-Ze.Cloning and sequencing of human soluble CD14[J].Letters in Biotechnology,2001,12(2):94-95,98. |
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| Authors: | BAI Jie WANG Wei YIN Jun WANG Zhan-dong WNAG Zhong-Ze |
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| Abstract: | Human soluble CD14 (sCD14)cDNA fragment was amplified from total RNA extracted from U937 cells by using RT PCR method, the fragment was cloned into pGEM T Plasmid. Identified by PCR, endonuclease digestion and sequencing. Sequencing confirmed sCD14 gene fragment was obtained and laid the foundation for further research on expression and biological activities. |
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| Keywords: | CD14 gene cloning sequencing |
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