Glutamate Release in Experimental Ischaemia of the Retina: An Approach Using Microdialysis |
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Authors: | P Louzada-Junior J J Dias W F Santos J J Lachat H F Bradford† J Coutinho-Netto |
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Institution: | Department of Biochemistry, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Brazil. |
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Abstract: | A rabbit eye model of neural ischaemia is described that uses an increased pressure in the anterior eye chamber to block the capillary supply to the retina. A microdialysis probe placed very close to the retinal surface was used to monitor release of amino acids during ischaemia. A large (two- to threefold) increase in the release of glutamate and O-phosphoserine (twofold), but not of six other amino acids monitored, occurred during initial ischaemia. During reperfusion after release of intraocular pressure, much larger (five- to 10-fold) increases in the release of these amino acids were observed. Parallel ischaemic retinal tissue damage was observed. This damage was prevented by ketamine applied locally via a superfusion needle, suggesting that glutamate released during ischaemia, and particularly during reperfusion, was responsible for cell death. |
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Keywords: | Retina Microdialysis Ischaemia Reperfusion Amino acids |
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