TGF-β1 induces efficient differentiation of human cardiomyocyte progenitor cells into functional cardiomyocytes in vitro |
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Authors: | Marie-Jos Goumans Teun P de Boer Anke M Smits Linda W van Laake Patrick van Vliet Corina HG Metz Tom H Korfage K Peter Kats Ron Hochstenbach Gerard Pasterkamp Marianne C Verhaar Marcel AG van der Heyden Dominique de Kleijn Christine L Mummery Toon AB van Veen Joost PG Sluijter Pieter A Doevendans |
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Institution: | Marie-José Goumans, Teun P. de Boer, Anke M. Smits, Linda W. van Laake, Patrick van Vliet, Corina H.G. Metz, Tom H. Korfage, K. Peter Kats, Ron Hochstenbach, Gerard Pasterkamp, Marianne C. Verhaar, Marcel A.G. van der Heyden, Dominique de Kleijn, Christine L. Mummery, Toon A.B. van Veen, Joost P.G. Sluijter,Pieter A. Doevendans, |
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Abstract: | The adult mammalian heart has limited regenerative capacity and was generally considered to contain no dividing cells. Recently, however, a resident population of progenitor cells has been identified, which could represent a new source of cardiomyocytes. Here, we describe the efficient isolation and propagation of human cardiomyocyte progenitor cells (hCMPCs) from fetal heart and patient biopsies. Establishment of hCMPC cultures was remarkably reproducible, with over 70% of adult atrial biopsies resulting in robustly expanding cell populations. Following the addition of transforming growth factor β, almost all cells differentiated into spontaneously beating myocytes with characteristic cross striations. hCMPC-derived cardiomyocytes showed gap-junctional communication and action potentials of maturing cardiomyocytes. These are the first cells isolated from human heart that proliferate and form functional cardiomyocytes without requiring coculture with neonatal myocytes. Their scalability and homogeneity are unique and provide an excellent basis for developing physiological, pharmacological, and toxicological assays on human heart cells in vitro. |
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