Detection of femtomolar quantities of the ganglioside GM1 on thin-layer chromatography plates by native choleratoxin and labeled antisera |
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Authors: | Anne-Brit Kolstø Otnaess Astrid Laegreid |
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Institution: | (1) Division of Molecular Cell Biology Department of Biology, University of Oslo, Blindern, PO Box 1050, N-0316 Oslo 3, Norway |
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Abstract: | After separation of gangliosides by thin-layer chromatography, femtomolar quantities of GM1 were detected by incubating the plate with native choleratoxin, followed by anticholeratoxin and species-specific labeled antiserum. Only stable reagents were involved when antiserum labeled with horseradish peroxidase was used. Native choleratoxin rather than iodinelabeled toxin ensured good reproducibility of the method. |
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