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Expression of a C3 plant Rubisco SSU gene in regenerated C4 Flaveria plants
Authors:Belinda Martineau  H Jane Smith  Caroline Dean  Pamela Dunsmuir  John Bedbrook  Laurens J Mets
Institution:(1) Department of Molecular Genetics and Cell Biology, University of Chicago, 60637 Chicago, IL, USA;(2) DNAP Corporation, 6701 San Pablo Avenue, 94608 Oakland, CA, USA;(3) Present address: Calgene, Inc., 1920 Fifth Street, 95616 Davis, CA, USA;(4) Present address: Competitive Research Grants Office, USDA, Room 323 Aerospace Bldg., 20251 Washington, D.C., USA;(5) Present address: John Innes Institute, Colney Lane, NR4 7UH Norwich, UK
Abstract:We report the successful transformation, via Agrobacterium tumefaciens infection, and regeneration of two species of the genus Flaveria: F. brownii and F. palmeri. We document the expression of a C3 plant gene, an abundantly expressed ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit gene isolated from petunia, in these C4 plants. The organ-specific expression of this petunia gene in Flaveria brownii is qualitatively identical to its endogenous pattern of expression.
Keywords:Agrobacterium tumefaciens  C4 photosynthesis  Flaveria  regeneration  ribulose bisphosphate carboxylase/oxygenase  transformation
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