In vitro selection of yellow passion fruit genotypes for resistance to <Emphasis Type="Italic">Fusarium</Emphasis> vascular wilt |
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Authors: | Patrícia Silva Flores Wagner Campos Otoni Onkar Dev Dhingra Sérgio Paulo Severo de Souza Diniz Telma Miranda dos Santos Claudio Horst Bruckner |
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Institution: | 1.Centro de Pesquisa Agroflorestal Acre,Empresa Brasileira de Pesquisa Agropecuária, Embrapa,Rio Branco,Brazil;2.Departamento de Biologia Vegetal,Universidade Federal de Vi?osa,Vi?osa,Brazil;3.Departamento de Fitopatologia,Universidade Federal de Vi?osa,Vi?osa,Brazil;4.Centro de Ciências Biológicas, Departamento de Bioquímica,Universidade Estadual de Maringá,Maringá,Brazil |
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Abstract: | Fusarium vascular wilt (caused by Fusarium oxysporum f. sp. passiflorae) is a limiting factor in the cultivation of yellow passion fruit (Passiflora edulis). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars
are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal
pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion
fruit were screened with different concentrations of either Fusarium oxysporum f. sp. passiflorae (FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige
and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation
between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen
were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments.
Observations indicated that roots were primary targets for attack by F. oxysporum. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy
was viable for accelerating breeding of passion fruit for resistance to the Fusarium vascular wilt. |
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