Targeted discovery of quantitative trait loci for resistance to northern leaf blight and other diseases of maize |
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| Authors: | Chia-Lin Chung Jesse Poland Kristen Kump Jacqueline Benson Joy Longfellow Ellie Walsh Peter Balint-Kurti Rebecca Nelson |
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| Institution: | (1) Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA;(2) Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, USA;(3) Department of Crop Science, North Carolina State University, Raleigh, NC 27695, USA;(4) U.S. Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA;(5) Present address: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, 10617, Taiwan;(6) Present address: U.S. Department of Agriculture, Agricultural Research Service, Department of Agronomy, Kansas State University, Manhattan, KS 66506, USA;(7) Present address: Department of Plant Pathology, The Ohio State University, Wooster, OH 44691, USA; |
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| Abstract: | To capture diverse alleles at a set of loci associated with disease resistance in maize, heterogeneous inbred family (HIF)
analysis was applied for targeted QTL mapping and near-isogenic line (NIL) development. Tropical maize lines CML52 and DK888
were chosen as donors of alleles based on their known resistance to multiple diseases. Chromosomal regions (“bins”; n = 39) associated with multiple disease resistance (MDR) were targeted based on a consensus map of disease QTLs in maize.
We generated HIFs segregating for the targeted loci but isogenic at ~97% of the genome. To test the hypothesis that CML52
and DK888 alleles at MDR hotspots condition broad-spectrum resistance, HIFs and derived NILs were tested for resistance to
northern leaf blight (NLB), southern leaf blight (SLB), gray leaf spot (GLS), anthracnose leaf blight (ALB), anthracnose stalk
rot (ASR), common rust, common smut, and Stewart’s wilt. Four NLB QTLs, two ASR QTLs, and one Stewart’s wilt QTL were identified.
In parallel, a population of 196 recombinant inbred lines (RILs) derived from B73 × CML52 was evaluated for resistance to
NLB, GLS, SLB, and ASR. The QTLs mapped (four for NLB, five for SLB, two for GLS, and two for ASR) mostly corresponded to
those found using the NILs. Combining HIF- and RIL-based analyses, we discovered two disease QTLs at which CML52 alleles were
favorable for more than one disease. A QTL in bin 1.06–1.07 conferred resistance to NLB and Stewart’s wilt, and a QTL in 6.05
conferred resistance to NLB and ASR. |
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