The effect of iron and/or zinc diet supplementation and termination of this practice on the antioxidant status of the reproductive tissues and sperm viability in rats |
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| Affiliation: | 1. Department of Physiology, Faculty of Pharmacy, Seville University, 41012, Seville, Spain;2. Faculty of Health Sciences, University Fernando Pessoa, Porto, Portugal;1. Department of Human Nutrition and Dietetics, Poznan University of Life Sciences, WojskaPolskiego St. 31, Poznan, 60-624, Poland;2. Department of Education and Obesity Treatment and Metabolic Disorders, Poznań University of Medical Sciences, ul. Szamarzewskiego 82/84, 60-569, Poznań, Poland;3. Department of Physiotherapy, Chair for Rehabilitation and Physiotherapy, Poznań University of Medical Sciences, 28 Czerwca 1956 r. St. 135/147, 61-545, Poznan, Poland;4. Department of Food Biotechnology and Microbiology, Poznan University of Life Sciences, Poznan, Poland;1. Department of Clinical Nutrition, Xin Hua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China;2. Department of Nutrition, Shanghai Jiao Tong University School of Medicine, Shanghai, China;3. Department of Epidemiology and Biostatistics, School of Public Health-Bloomington, Indiana University, Bloomington, IN, USA;4. Department of Obstetrics and Gynecology and Department of Epidemiology, Columbia University Irving Medical Center, New York, NY, USA;5. Health Sciences Center, School of Medicine, University of New Mexico, Albuquerque, NM, USA;6. Division of Preventive Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA |
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| Abstract: | AimsThe aim of this study was to investigate the effect of iron or/and zinc supplementation and termination of this treatment on the antioxidant defence of the male reproductive system and sperm viability in rats.MethodsThe study consisted of 3 stages: I) 4-week adaptation to the diets (C-control or D-iron deficient); II) 4-week iron and/or zinc supplementation (10-times more than in the C diet of iron: CSFe, DSFe; zinc: CSZn, DSZn; or iron and zinc: CSFeZn, DSFeZn; and III) 2-week post-supplementation period (the same diets as during stage I). Parameters of antioxidant status (total antioxidant capacity and SOD, GPx, and CAT activiy), oxidative damage (lipid and protein peroxidation), and sperm viability were measured.ResultsSimultaneous iron and zinc supplementation compared to iron supplementation (CSFeZn vs CSFe) increased SOD activity in the testes and decreased the level of malondialdehyde in the epididymis after stage II, and increased the percentage of live sperm after stage III. After discontinuation of the iron and zinc supplementation and a return to the control diet, the following was observed a decrease of SOD activity in the testes and GPx activity in the epididymis, and a increase malondialdehyde concentration in prostates. After stage III, in DSFeZn vs DSFe rats, an increase of SOD and CAT activity in the epididymis was found.ConclusionZinc supplementation simultaneous with iron may protect the male reproductive system against oxidative damage induced by high doses of iron and may have a beneficial effect on sperm viability. The effect of this supplementation was observed even two weeks after the termination of the intervention. |
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| Keywords: | Iron Zinc Supplementation Antioxidant status Sperm viability Reproductive tissues |
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