A simple dilute-and-shoot procedure for the determination of platinum in human pleural effusions using HR-CS GF-AAS |
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| Institution: | 1. Department of Public Health, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic;2. Department of Respiratory Diseases, University Hospital Brno and Faculty of Medicine, Masaryk University, Jihlavská 20, 625 00, Brno, Czech Republic;3. Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic;4. Institute of Analytical Chemistry of the Czech Academy of Sciences, Veve?í 97, 602 00, Brno, Czech Republic;5. Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University in Bratislava, Slovakia;1. Department of Physiology, School of Sport Sciences, University of Extremadura, Avenida de la Universidad s/n, 10003 Cáceres, Extremadura, Spain;2. Department of Sport Science, Faculty of Education, Pontifical University of Salamanca, C/Henry Collet, 52–70, CP: 37007 Salamanca, Spain;3. Movement, Brain and Health (MOBhe) Research Group, Center of Higher Education Alberta Giménez, affiliated to Comillas Pontifical University, Costa de Saragossa 16, 07013 Palma, Mallorca, Balearic Islands, Spain;4. Faculty of Language and Education, University of Nebrija, Campus La Berzosa, Calle del Hostal, 28248, Hoyo de Manzanares, Madrid, Spain;1. Center for Advanced Studies and Technology (C.A.S.T.), “G. d''Annunzio” University of Chieti-Pescara, Via Luigi Polacchi 11, Chieti, I-66100, Italy;2. Department of Physics, Università Degli Studi di Milano, Via Celoria 16, Milano, I-20133, Italy;3. LASA, Department of Physics, Università Degli Studi di Milano and INFN-Milano, Via F.lli Cervi 201, Segrate, MI, I-20090, Italy;4. Institute of Clinical Immunotherapy and Advanced Biological Treatments, Piazza Pierangeli 1, Pescara, Italy;5. Rectorate of Leonardo da Vinci Telematic University, Largo San Rocco 11 Torrevecchia, Teatina, CH, Italy;6. Department of Medicine and Aging Sciences, “G. d’Annunzio” University of Chieti-Pescara, via Luigi Polacchi 11, Chieti, I-66100, Italy;1. Unitat de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d’Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, C. Sant Joan s/n, 43201, Reus, Spain;2. Department of Radiation Oncology, Hospital Universitari de Sant Joan, Institut d’Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Av. Doctor Josep Laporte 2, 43204, Reus, Spain;1. Department of Pediatrics, All India Institute of Medical Sciences, Jodhpur, 342005, India;2. WQMG/DEST Division, Defence Research and Development Organization, Jodhpur, 342011, India;3. Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Jodhpur, 342005, India;4. Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, 342005, India;5. Department of Pediatrics, All India Institute of Medical Sciences, Jodhpur, 342005, India;1. Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi, 030001, China;2. Key Lab of Environmental Hazard and Health of Shanxi Province, Shanxi Medical University, Taiyuan, Shanxi, 030001, China;3. Key Lab of Cellular Physiology of Education Ministry, Shanxi Medical University, Taiyuan, Shanxi, 030001, China;4. Department of Anatomy, Shanxi Medical University, Taiyuan, Shanxi, 030001, China |
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| Abstract: | BackgroundHigh-resolution continuum source AAS is an emerging technique for the determination of trace elements in clinical analysis. We aimed to develop a method for the direct determination of platinum (Pt) in pleural effusions that could deepen the understanding of the dynamics of intrapleural Pt concentration during cytostatic therapy.Materials and methodsSamples were collected by thoracic drainage from five patients with lung cancer undergoing platinum based chemotherapy. A simple dilute-and-shoot method for Pt determination in the pleural effusions was developed. Ashing of the sample in an oxygen flow in a graphite tube allowed for direct analysis without prior mineralization. The trueness of the method was verified using an independent technique (ICP-MS). As platinum derivatives are only active in its free form (not bound to proteins), Pt in samples was further divided into free and protein-bound forms by means of ultrafiltration.ResultsUsing the proposed method, Pt contents (free and total) were determined in samples collected at different times after the intravenous application of the Pt derivative. The concentration of total Pt reached values of up to 5,000 μg/L and different patterns of its dynamics in intrapleural fluid were observed.ConclusionsThe developed method enables the fast and simple determination of Pt in biological fluids. It may be applied on a large scale to improve the understanding of Pt dynamics during cytostatic therapy, and also to determine the optimal timing of both thoracic drainage and administration of systemic chemotherapy. |
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| Keywords: | Lung cancer Platinum Pleural effusion HR-CS GF-AAS |
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