Cloning and in vitro characterization of dTDP-6-deoxy-l-talose biosynthetic genes from Kitasatospora kifunensis featuring the dTDP-6-deoxy-l-lyxo-4-hexulose reductase that synthesizes dTDP-6-deoxy-l-talose |
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Authors: | Suman Karki |
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Institution: | Department of Biological Science, Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Republic of Korea |
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Abstract: | Kitasatospora kifunensis, the talosin producer, was used as a source for the dTDP-6-deoxy-l-talose (dTDP-6dTal) biosynthetic gene cluster, serving as a template for four recombinant proteins of RmlAKkf, RmlBKkf, RmlCKkf, and Tal, which complete the biosynthesis of dTDP-6dTal from dTTP, α-d-glucose-1-phosphate, and NAD(P)H. The identity of dTDP-6dTal was validated using 1H and 13C NMR spectroscopy. K. kifunensistal and tll, the known dTDP-6dTal synthase gene of Actinobacillus actinomycetemcomitans origin, have low sequence similarity and are distantly related within the NDP-6-deoxy-4-ketohexose reductase family, providing an example of the genetic diversity within the dTDP-6dTal biosynthetic pathway. |
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Keywords: | l-talose" target="_blank">dTDP-6-Deoxy-l-talose Kitastospora kifunensis Biosynthetic gene cluster l-lyxo-4-hexulose reductase" target="_blank">dTDP-6-Deoxy-l-lyxo-4-hexulose reductase |
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