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Cloning of a Vero toxin (VT2) gene from a VT2-converting phage isolated from Escherichia coli 0157: H7 |
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| Authors: | Takashi Yutsudo Hisao Kurazono Chihiro Sasakawa Masanosuke Yoshikawa Makoto Iwaya Tae Takeda Yoshifumi Takeda |
| Institution: | Department of Bacterial Infection, Institute of Medical Science, University of Tokyo, Japan;Department of Bacteriology, Institute of Medical Science, University of Tokyo, Japan;Department of Infectious Diseases, National Children's Medical Research Center, Tokyo, Japan |
| Abstract: | Abstract A Vero toxin (VT2 or Shiga-like toxin II)-converting phage was isolated from Escherichia coli 0157: H7 strain J-2. Nontoxigenic E. coli C600 produced VT2 when lysogenized with the toxin-converting phage. Eco RI fragments of the phage DNA were ligated with Eco RI-digested pBR322 or pUC118 and were transformed into E. coli MC1061 or MV1184. Transformants exhibiting VT2 production commonly contained a 4.6 kb Eco RI fragment. It was found that a 2.3 kb Kpn I- Sph I fragment coded VT2 production and that this fragment hybridized weakly with the 2.1 kb fragment encoding VT1. |
| Keywords: | VT2 Cloning of VT2 gene Escherichia coli 0157 H7 |