| 多个抗虫基因转化水稻两用系培矮—64S |
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| 引用本文： | 范钦 许新萍. 多个抗虫基因转化水稻两用系培矮—64S[J]. 实验生物学报, 2002, 35(1): 42-46 |
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| 作者姓名： | 范钦 许新萍 |
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| 摘 要： | 应用基因枪法对水稻两用系培矮－64S进行了转化。质粒载体pKC-3串联了三个抗虫基因，将其转化成熟胚诱导形成的愈伤组织，共获得33株转基因植株。分别对R0代植株不同的基因进行PCR及Southern blot分析，并对R1代植株进行了PCR分析。结果表明，三个抗虫基因均已整合到水稻基因组并获得稳定遗传。
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| 关 键 词： | 水稻 基因枪法 抗虫基因 培矮－64S 两用系 基因转化 |
Transferring of multiple insect-resisting genes into two-line genetic male sterile rice Pei'ai 64S by particle bombardment] |
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| Qin Fan,Xin Ping Xu,Bao Jian Li. Transferring of multiple insect-resisting genes into two-line genetic male sterile rice Pei'ai 64S by particle bombardment][J]. Acta Biologiae Experimentalis Sinica, 2002, 35(1): 42-46 |
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| Authors: | Qin Fan Xin Ping Xu Bao Jian Li |
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| Affiliation: | Life Science Research Center of Zhongshan University, Guangzhou 510275. |
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| Abstract: | Transformation of two-line genetic male sterile indica rice variety Pei'ai 64S was conducted by particle bombardment. Three insect-resisting genes ligased into plasmid vector pKC-3 was introduced into calli derived from mature embryo and a total of 33 transgenic plants had been obtained. The PCR and Southern blot analysis of each different gene in R0 plants and also PCR analysis of each different gene in R1 plants showed the integration of three insect-resisting genes into the genome of transgenic plants which could stably pass down. |
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