| The effect of external Ca^2＋ and Ca^2＋—channel modulators on red—light—induced swelling of protoplasts of Phaseolus radiatus L. |
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| 引用本文： | LONGCHENG XIAOJINGWANG 等. The effect of external Ca^2＋ and Ca^2＋—channel modulators on red—light—induced swelling of protoplasts of Phaseolus radiatus L.[J]. Cell research, 1998, 8(1): 41-50 |
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| 作者姓名： | LONGCHENG XIAOJINGWANG 等 |
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| 作者单位： | [1]DepartmentofBiology,southChinaNormalUniversity,Guangzhou510631,China [2]DepartmentofBiology,southChinaNo |
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| 摘 要： | Red-light-induced swelling of the protoplasts isolated from hypocotyl of etiolated mung bean(Phaseolus radiatusL.)was observed only when Ca^2 ions were present in the medium.The optimal CaCl2 concentration was 250μM,Swlling response declined when Ca^2 was supplied into the medium after red light irradiation.The Ca^2 -chelator EGTA eliminated the red-light-induced swelling and 45Ca^2 accumulation in the protoplasts.In conltrast,A23187,a Ca^2 -ionophore,could mimic the effect of red light in darkness.These results indicate that Ca^2 may play a role in light signal transduction.In addition,swelling response was prevented by TFP and CPZ(both are CaM antagonists),implying the involvement of CaM in red-light-induced and Ca^2 -dependent protoplast swelling.
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| 关 键 词： | 菜豆 原生质体 红光诱发溶胀 钙离子 钙通道 调控代谢物 |
The effect of external Ca~(2 ) and Ca~(2 )-channel modulators on red-light-induced swelling of protoplasts of Phaseolus radiatus L. |
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| LONG CHENG,XIAO JING WANG, RUI CHI PAN. The effect of external Ca~(2 ) and Ca~(2 )-channel modulators on red-light-induced swelling of protoplasts of Phaseolus radiatus L.[J]. Cell research, 1998, 8(1): 41-50 |
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| Authors: | LONG CHENG XIAO JING WANG RUI CHI PAN |
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| Abstract: | |
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| Keywords: | Calcium ions red light protoplast swelling ~(45)Ca~(2 ) accumulation Phaseolus radiatus |
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