新霉胺通过抑制血管生成素活性抑制人黑色素瘤细胞增殖、迁移和侵润

新霉胺（neamine）是一种无毒的新霉素（neomycin）降解产物；已有研究证明,其可抑制血管生成素（angiogenin，ANG）诱导的内皮细胞血管生成作用，阻滞异种移植的人结肠腺癌在裸鼠的生长.本研究证明,新霉胺对人黑色瘤细胞株A375的细胞增殖、迁移和侵润作用.MTT法及软琼脂培养显示，新霉胺可明显抑制A375细胞的增殖和集落形成能力. Transwell试验证明，新霉胺可阻滞A375细胞，乃至血管生成素诱导的A375细胞的迁移和侵润能力.此外，免疫荧光揭示新霉胺可阻断血管生成素的核转位，从而抑制血管生成素诱导的A375细胞增殖.上述结果提示，新霉胺可通过抑制血管生成素的核转位，从而抑制肿瘤细胞增殖、迁移和侵润.鉴于与新霉素比较，新霉胺毒性小，因此新霉胺可望作为黑色素瘤治疗的先导药物，颇具开发前景和潜力.

Neamine Inhibits A375 Human Melanoma Cell Proliferation,Migration, and Invasion by Blocking Angiogenin Activity

Neamine, a nontoxic degradation product of neomycin, has been shown to inhibit angiogenin induced angiogenesis in endothelial cells and xenograft growth of human colon adenocarcinoma in athymic mice. Herein, we demonstrated the effects of neamine on cell proliferation, migration and invasion of human melanoma cell A375. MTT and soft agar assays showed that neamine markedly blocked the cellular proliferation of A375 cells and its colony formation. Transwell assays revealed that neamine significantly inhibited the migration and invasion of A375 cells. Moreover, neamine blocked the nuclear translocation of angiogenin in immunofluorescence staining and prevented angiogenin induced cellular proliferation as well as angiogenin induced migration and invasion of A375 cells. These data indicated that neamine might block cancer cell proliferation, migration and invasion, probably due to inhibition of nuclear translocation of angiogenin. Since the toxicity of neamine is much less than neomycin, it may serve as a lead agent for the development of human melanoma therapeutics