New aspects in the synthesis and secretion of lysozyme by cultured human monocyte cell lines |
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Authors: | Racha Helal Matthias F Melzig |
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Institution: | 1.Department of Pharrmaceutical Biology, Institute of Pharmacy,Free University of Berlin,Berlin,Germany |
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Abstract: | The main purpose of this study was to investigate lysozyme synthesis and secretion in three human monocyte cell lines: U-937,
HL-60, and THP-1, using sensitive fluorescence-based assay of lysozyme activity. PMA and hIFN-γ were evaluated for inducing
lysozyme activity. Using well-defined cell lines from the cell culture collection, no lysozyme activity could be detected
in the cultured U-937 cells either with or without addition of the inducing factors. These data suggested, contrary to previous
reports, that U-937 cell line cannot synthesize or secrete active lysozyme. THP-1 and HL-60 cells were proved to produce enzymatically
active lysozyme in increasing amounts with the time course. PMA and hIFN-γ had no significant inducing effect on the production
or the release of active lysozyme in THP-1 and HL-60 cells. We showed inhibiting effect of PMA and hIFN-γ on the lysozyme
activity, particularly in HL-60 cell line. |
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