<Emphasis Type="Italic">In vitro</Emphasis> propagation of <Emphasis Type="Italic">Chimonanthus praecox</Emphasis> (L.), a winter flowering ornamental shrub |
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Authors: | B Kozomara B Vinterhalter Lj Radojevi? D Vinterhalter |
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Institution: | (1) Plant Physiology Department, Institute for Biological Research, S. Stanković, Belgrade University, Bulevar despota Stefana 142, 11060 Belgrade, Serbia |
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Abstract: | A procedure for the micropropagation of Chimonanthus praecox (L) Link, wintersweet, has been developed using buds from adult trees excised in spring. Shoot cultures established on Murashige
and Skoog (1962) medium supplemented with 0.5 mg l−1 6-benzyladenine (BAP) and 0.1 mg l−1 indole-3-butyric acid (IBA) were difficult to maintain in vitro through extended periods of time due to browning of the medium, shoot and leaf necrosis, and hyperhydricity. A treatment
combining the use of 0.1% w/v activated charcoal and addition of a double phase agar-solidified/liquid medium improved propagation, enabling a successful
in vitro propagation scheme to be developed. Optimal shoot multiplication occurred on medium containing 0.5 mg l−1 BAP, and rooting on medium with 2.0 mg l−1 IBA for 7 d, followed by transfer to hormone-free medium. Rooted plantlets were easily acclimated in a glasshouse and replanted
and cultured outdoors. |
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Keywords: | Wintersweet Micropropagation Chimonanthus praecox Activated charcoal Double phase culture medium |
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