| 褐煤强化产甲烷菌群的群落分析及条件优化 |
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| 引用本文： | 占迪,何环,廖远松,赵尚明,姚菁华,肖栋,唐俊,陶秀祥. 褐煤强化产甲烷菌群的群落分析及条件优化[J]. 微生物学报, 2018, 58(4): 684-698 |
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| 作者姓名： | 占迪 何环 廖远松 赵尚明 姚菁华 肖栋 唐俊 陶秀祥 |
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| 作者单位： | 中国矿业大学化工学院, 煤炭加工与高效洁净利用教育部重点实验室, 江苏 徐州 221116,中国矿业大学化工学院, 煤炭加工与高效洁净利用教育部重点实验室, 江苏 徐州 221116,中国矿业大学化工学院, 煤炭加工与高效洁净利用教育部重点实验室, 江苏 徐州 221116,中国矿业大学化工学院, 煤炭加工与高效洁净利用教育部重点实验室, 江苏 徐州 221116,中国矿业大学化工学院, 煤炭加工与高效洁净利用教育部重点实验室, 江苏 徐州 221116,中国矿业大学煤炭资源与安全开采国家重点实验室, 江苏 徐州 221116,中国矿业大学安全学院, 江苏 徐州 221116,中国矿业大学化工学院, 煤炭加工与高效洁净利用教育部重点实验室, 江苏 徐州 221116 |
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| 基金项目： | 国家自然科学基金（51404259）；江苏省自然科学基金（BK20150181） |
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| 摘 要： | 【目的】以白音华褐煤为底物,利用从我国多地煤矿及污水处理厌氧罐中富集-混合-驯化得到的高效混合菌群进行产气,分析其群落组成并优化产气条件。【方法】采用Miseq高通量测序分析混合菌群结构,通过Plackett-Burman(P-B)和Box-Behnken(B-B)试验对褐煤产气影响因素和条件进行筛选和优化。【结果】本源和外源微生物样本混合样品(HN+MD+WT)经驯化后菌群产气效率最高。该样品菌群中细菌群落多样性丰富,以变形菌门的脱硫弧菌属Desulfovibrio(15.07%)、拟杆菌门的屠场杆状菌属Macellibacteroides(14.6%)、厚壁菌门的梭菌属Clostridiaceae(9.77%)、互营菌门的脱硫代硫酸盐弧菌属Dethiosulfovibrio(8.76%)以及热袍菌门Oceanotoga属(8.66%)为主。古菌全部为广古菌门,其群落多样性则较为单一,其中甲烷卵圆形菌属Methanocalculus(80.28%)占据绝对优势。Plackett-Burman(P-B)试验结果表明温度、CoCl_2添加量和NiCl_2添加量是影响褐煤产气的关键因素;Box-Behnken(B-B)试验结果表明最优产气条件为:温度36°C,CoCl_2添加量0.17 g/L,NiCl_2添加量0.02 g/L,最优条件下褐煤累计产甲烷量(周期20 d)达到159.33μmol/g。【结论】经过驯化可以得到高效的产气菌群,优化培养条件可使产气效率明显提高。
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| 关 键 词： | 褐煤 生物强化产气 微生物群落分析 产气条件优化 |
| 收稿时间： | 2017-09-15 |
| 修稿时间： | 2017-12-10 |
Community structure analysis of methanogenic flora and optimization for bioaugmentation methane generation from lignite |
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| Di Zhan,Huan He,Yuansong Liao,Shangming Zhao,Jinghua Yao,Dong Xiao,Jun Tang and Xiuxiang Tao. Community structure analysis of methanogenic flora and optimization for bioaugmentation methane generation from lignite[J]. Acta microbiologica Sinica, 2018, 58(4): 684-698 |
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| Authors: | Di Zhan Huan He Yuansong Liao Shangming Zhao Jinghua Yao Dong Xiao Jun Tang Xiuxiang Tao |
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| Affiliation: | Key Laboratory of Coal Processing & Efficient Utilization, Ministry of Education, School of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China,Key Laboratory of Coal Processing & Efficient Utilization, Ministry of Education, School of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China,Key Laboratory of Coal Processing & Efficient Utilization, Ministry of Education, School of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China,Key Laboratory of Coal Processing & Efficient Utilization, Ministry of Education, School of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China,Key Laboratory of Coal Processing & Efficient Utilization, Ministry of Education, School of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China,State Key Laboratory of Coal Resources and Safety Mining, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China,School of Safety Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China and Key Laboratory of Coal Processing & Efficient Utilization, Ministry of Education, School of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, Jiangsu Province, China |
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| Abstract: | |
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| Keywords: | lignite bioaugmentation methane production microbial community analysis condition optimization of biomethane generation |
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