THE NATURE OF THE TWO PROTEINS OF BRAIN SPECIFIC ANTIGEN 14-3-2 |
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| Authors: | Elisabeth Bock Lynne Fletcher C C Rider C B Taylor |
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| Institution: | The Protein Laboratory, University of Copenhagen, 34, Sigurdsgade, K-2200 Copenhagen, Denmark;The Department of Biochemistry, University of Sheffield, Sheffield S10 2TN, U.K. |
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| Abstract: | The three isoenzymes of rat brain enolase (2-phospho d -glycerate hydrolase EC 4.2.1.11.) χχ, χγ and γγ were separated by ion-exchange chromatography and were tested for reaction with an antiserum against brain specific antigen 14-3-2. This monospecific antiserum affects the enolase activity of only the χγ and γγ isoenzymes. Immunoelectrophoretic experiments show that the two proteins which react as 14-3-2 both contain γ enolase subunits, and one of these also contains χ enolase subunits. It is concluded that the 14-3-2 antigen and the γ enolase subunit are identical, and that the two proteins which react immunologically as 14-3-2 are the χγ and γγ enolase isoenzymes. |
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