In vivo preparation of (32P) adenosine 3',5'-cyclic monophosphate |
| |
Authors: | H Yamazaki K Potter G Chaloner-Larsson |
| |
Institution: | Department of Biology, Carleton University, Ottawa, Ontario, K1S 5B6, Canada |
| |
Abstract: | A simple method for the preparation of 32P]adenosine 3′,5′-cyclic monophosphate (cyclic AMP) is described. A culture of Escherichia coli mutant deficient in cyclic AMP receptor protein is incubated with 32P]orthophosphate of known specific activities (up to 4000 Ci/mole) for several cell doublings. 1012 cells of this mutant excrete approximately 1.4 μmoles of cyclic AMP/hr. The extracellular cyclic AMP can be purified by adsorption to charcoal, chromatography on an alumina plate, and paper chromatography. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|