26种滇产植物提取物的体外抗耐药菌作用研究

研究26种云南滇东南产植物80%乙醇提取物的体外抗耐药菌作用。制备植物的醇提物,用琼脂打孔法对其进行抗菌活性的筛选。通过微量稀释法测定提取物的最低抑菌浓度minimum inhibitory concentration(MIC)]、最低杀细菌浓度minimum bactericidal concentration(MBC)]及最低杀真菌浓度minimum fungicidal concentration(MFC)]。结果表明:26种提取物中有9种对金黄色葡萄球菌(简称金葡菌)、大肠埃希菌、铜绿假单胞菌、白色念珠菌有不同程度的抑制活性;其中以红花荷、马蹄参、百花贝母兰、二色大包兰和光序肉实树提取物对耐甲氧西林金葡菌的抑菌作用最强,其MIC/MBC(mg/L)范围分别为512/2048～>2048,512/2048～>2048,256-512/2048～>2048,512～1024/1024～>2048和512～1024/>2048。另外,肋果茶提取物对铜绿假单胞菌及白色念珠菌有较好的抑制作用,其对铜绿假单胞菌及其耐药菌株的MIC/MBC为512～2048/>2048mg/L;对白色念珠菌及其耐药菌株的MIC/MFC均为2048/>2048mg/L。26种植物提取物对大肠埃希菌的抑制作用均较弱。

Antimicrobial activities of 26Yunnan plants extracts against clinical multi-drug resistant pathogens in vitro

To determine the in vitro antimicrobial activities of 80%ethanol extracts from 26plants originated in southeast of Yunnan.The ethanol extracts of the collected plant samples were subjected to screen of the antibacterial and antifungal activity by the agar hole diffusion test.The minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) or minimum fungicidal concentration(MFC) were determined by serial dilution with a standard broth microdilution method.The results showed that 9of the 26extracts were active with different potency against strains of Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Candida albicans.The most active extracts against Staphylococcusa ureus and methicillin-resistant S.aureus(MRSA) were fromRhodoleiap arvipetala Tong,Diplopanax stachyanthus Hand.-Mazz,Sarcosperma kachinense Exell var.simondii Lam.et Royen,Coelogyne leucantha W.W.Smith and Sunipia bicolor.Their MICs /MBCs(mg /L) were 512 /2048->2048,512 /2048->2048,256-512 /2048->2048,512-1024 /1024->2048and 512-1024 />2048,respectively.Sladenia celastrifolia Kurz showed strong activity against P.aeruginosaand C.albicans,and the MICs /MBCs(MFCs) were 512-2048 />2048and 2048 />2048mg /L,respectively.All these plants showed weak inhibition against Escherichia coli.