Distribution and organization of a tandemly repeated 352-bp sequence in the oryzae family |
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| Authors: | A De Kochko M C Kiefer F Cordesse A S Reddy M Delseny |
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| Institution: | (1) Laboratory of Plant Physiology and Molecular Biology URA 565 du CNRS, Avenue de Villeneuve, F-66860 Perpignan-Cédex, France;(2) Present address: Department of Biology, Washington University, 63130 St. Louis, MO, USA;(3) Present address: Department of Biology, Texas A&M University, 77843-3258 College Station, TX, USA |
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| Abstract: | Summary A 352-bp EcoRI fragment from rice DNA was cloned and shown to be a member of a tandem repeat. Sequence determination revealed homologies with human alpha satellite DNA and maize knob heterochromatin specific repeat. This 352-bp sequence is highly specific for the AA genome of rice. However, copy number and sequence organization are variable, depending on the accession analyzed. Several examples of amplification were observed in O. rufipogon and O. longistaminata. Use of resolutive polyacrylamide gel electrophoresis and 4-bp cutter enzymes allowed one to distinguish between the Indica and Japonica subtypes of O. sativa. The same method also discriminates between two groups of O. rufipogon, the presumed ancestor of O. sativa, suggesting that the present day Indica and Japonica subtypes originated independently from two O. rufipogon distinct populations. |
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| Keywords: | Cultivated rice Wild rice RFLP Satellite DNA Evolution |
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