Composition of essential oil in genetically transformed roots of <Emphasis Type="Italic">Ruta graveolens</Emphasis> |
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Authors: | I N Kuzovkina Sz Szarka É Héthelyi E Lemberkovics É Szöke |
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Institution: | 1.Timiryazev Institute of Plant Physiology,Russian Academy of Sciences,Moscow,Russia;2.Department of Pharmacognosy,Semmelweis University,Budapest,Hungary |
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Abstract: | The composition of essential oil obtained by hydrodistillation from genetically transformed roots of common rue (Ruta graveolens L.) was analyzed. Using gas chromatography and complex gas chromatography-mass spectrometry, it was established that the
major component of rue essential oil was a root-specific sesquiterpene geijerene comprising 67% of total amount of volatile
compounds. In essential oil of cultured rue roots, furocoumarins characteristic of intact plant roots were found, viz. osthole,
halepensin, and rutacultin. The content of essential oil in genetically transformed rue roots was 0.23% of root dry weight,
which is comparable with that in the roots of intact plants. The long-term maintenance in the in vitro cultured rue roots
of a capability for the synthesis of essential oil major components characteristic of intact plants allows their usage for
studying the physiological activity of these volatile compounds and their putative role in the plant root interaction in biocenoses. |
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