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Exploration of the Genomic Diversity and Core Genome of the Bifidobacterium adolescentis Phylogenetic Group by Means of a Polyphasic Approach
Authors:Sabrina Duranti  Francesca Turroni  Christian Milani  Elena Foroni  Francesca Bottacini  Fabio Dal Bello  Alberto Ferrarini  Massimo Delledonne  Douwe van Sinderen  Marco Ventura
Institution:aLaboratory of Probiogenomics, Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, Parma, Italy;bAlimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Cork, Ireland;cSacco Srl, Cadorago, Italy;dFunctional Genomic Center, Department of Biotechnology, University of Verona, Verona, Italy
Abstract:In the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., Bifidobacterium adolescentis, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16S rRNA gene and 16S-23S internal transcribed spacer (ITS) sequences, pulsed-field gel electrophoresis (PFGE), and comparative genomic hybridization (CGH) assays.
Keywords:
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