番茄AT-hook基因家族的鉴定及胁迫条件下的表达分析

AT-hook蛋白家族在植物生长发育、器官构建及逆境胁迫和激素信号应答中发挥重要作用。本研究在番茄基因组范围内,利用生物信息学方法对番茄AT-hook基因家族的成员、分布、结构和功能进行分析。结果表明,番茄AT-hook家族包含32个成员,分为3种类型,其中类型Ⅰ含有13个成员;遗传进化分析表明番茄AT-hook基因成员与拟南芥家族基因具有相似分类。利用实时荧光定量PCR对番茄32个基因开展组织表达分析,结果表明AT-hook基因具有表达差异,主要在根和花中表达较高。氧化胁迫分析结果表明,32个基因受ABA、SA、盐、高温和低温诱导表达,其中部分基因显著上调或下调表达,很可能参与了番茄逆境胁迫条件下的防御应答反应。本研究结果将为番茄AT-hook家族基因的深入研究提供依据,为进一步解析番茄AT-hook基因的功能奠定基础。

Genome-wide Identification and Expression Analysis in Oxidative Stress of AT-hook Gene Family in Tomato

AT-hook proteins play an important role in regulation of eukaryotic gene expression, and implicated in plant development, organ formation, stress response, and hormonal signaling. In this study, our analysis revealed the presence of 32 genes encoding AT-hook proteins in the tomato genome, the distribution on the chromosome, the structure and function of proteins were analyzed. AT-hook proteins could be classified into 3 type based on phylogenetic analysis. , 13 of them belong to Type I, and other members have the similar classification with Arabidopsis thaliana, which was supported with organization of predicted conserved putative motifs in AT-hook proteins. Real-time fluorescence quantitative PCR were used to gain insights into the function of AT-hook gene family members during various stages of development and in response to abiotic stress and phytohormones. Some of the AHL genes were expressed in specific organs such as roots and flowers; 32 SlAHL genes showed distinct expression patterns in response to stresses of salt, SA, ABA, high temperature and cold, the expressions of several genes were significantly up/down regulated, implying that these members might participate in regulating the defense response against abiotic stresses. The results will provide a very useful reference for cloning and functional analysis of each member of AT-hook gene family in Solanaceae crops.