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Comparison of Aberrant Methylation of CpG Islands in the p16/CDKN2A and p14/ARF Promoters in Non-Small Cell Lung Cancer and Acute Lymphoblastic Leukemia |
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| Authors: | Zemlyakova V. V. Strel'nikov V. V. Zborovskaya I. B. Balukova O. V. Maiorova O. A. Vasil'ev E. V. Zaletaev D. V. Nemtsova M. V. |
| Affiliation: | (1) Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moscow, 115478, Russia;(2) Institute of Molecular Medicine, Sechenov Medical Academy, Ministry of Health of the Russian Federation, Moscow, 119992, Russia;(3) Institute of Carcinogenesis, Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow, 115478, Russia;(4) Institute of Children's Hematology, Ministry of Health of the Russian Federation, Moscow, 117531, Russia |
| Abstract: | Multiplex methylation-sensitive (MSe-PCR) and methylation-specific (MSp-PCR) PCRs were used to detect aberrant methylation of CpG islands in the promoter regions and first exons of p16/CDKN2A and p14/ARF in non-small cell lung cancer (NSCLC, 54 specimens) and B-cell acute lymphoblastic leukemia (B-ALL, 61 specimens). A difference in CpG methylation was observed for individual specimens and for the two malignancies. A high methylation frequency of the first exon of p16/CDKN2A was detected both in NSCLC (68%) and in B-ALL (55%). The CpG island of the p14/ARF first exon proved to be nonmethylated in both malignancies. Particular CpG-rich fragments were examined in the p16/CDKN2A and p14/ARF promoters. It was shown that methylation frequency can differ between the 5 regions of one promoter. The sensitivity was compared for MSe-PCR and MSp-PCR, which are commonly employed in methylation analysis. |
| Keywords: | p16/CDKN2A p14/ARF CpG island promoter region methylation-sensitive PCR methylation-specific PCR methylation-specific sequencing non-small cell lung cancer B-cell acute lymphoblastic leukemia acute lymphoblastic leukemia |
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