(1) Department of Food Science and Biotechnology, Kyonggi University, Suwon, 443-760, South Korea;(2) Nong Shim Co., LTD., Kunpo, 435-713, South Korea
Abstract:
A novel transglycosylation reaction from sucrose to l-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable l-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-α-d-glucopyranosyl-l-ascorbic acid by LC-MS/MS analysis.