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Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum
Authors:Taeyeon Kwon  Cheong Tae Kim  Jong-Hoon Lee
Affiliation:(1) Department of Food Science and Biotechnology, Kyonggi University, Suwon, 443-760, South Korea;(2) Nong Shim Co., LTD., Kunpo, 435-713, South Korea
Abstract:A novel transglycosylation reaction from sucrose to l-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable l-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-α-d-glucopyranosyl-l-ascorbic acid by LC-MS/MS analysis.
Keywords:  font-variant:small-caps"  >l-Ascorbic acid   Bifidobacterium longum   2-O  -  font-variant:small-caps"  >d-Glucopyranosyl-  font-variant:small-caps"  >l-ascorbic acid  Sucrose phosphorylase  Transglucosylation
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