Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels |
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Authors: | Silva Joana Carvalho Ana Sofia Vitorino Rui Domingues Pedro Teixeira Paula Gibbs Paul |
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Institution: | Escola Superior de Biotecnologia, Universidade Catolica Portuguesa, Rua Dr. Antonio Bernardino de Almeida, 4200-072 Porto, Portugal. |
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Abstract: | Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming. |
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