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Blockade of preS2 down-regulates the apoptosis of HepG2.2.15 cells induced by TRAIL |
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| Authors: | Liang Xiaohong Qu Zhonghua Zhang Zhiyong Du Juan Liu Yugang Cui Min Liu Hua Gao Lifen Han Lihui Liu Suxia Cao Lili Zhao Peiqing Sun Wensheng |
| Affiliation: | a Institute of Immunology, School of Medicine, Shandong University, Jinan 250012, China b Department of Pathophysiology, Shandong University, Jinan, China c Department of Physiology, Shandong University, Jinan, China d Qianfo Mountain Hospital, Jinan 250014, China |
| Abstract: | The TNF-related apoptosis-inducing ligand (TRAIL) has recently been implicated in the death of hepatocytes under infectious but not normal conditions. Our previous studies showed that both the whole HBV genome and its HBx protein enhanced TRAIL-induced hepatocyte apoptosis. We report here that preS2-containing viral proteins are also potent regulators of TRAIL-induced apoptosis. HBV-transfected hepatoma cell line, HepG2.2.15, pretreated with antisense oligonucleotide against preS2 gene, showed a lower sensitivity towards TRAIL-induced apoptosis. However, this effect might not be related with the expression level of TRAIL receptors. These results establish that besides HBx, preS2 viral proteins are also involved in enhancing TRAIL-induced hepatocyte apoptosis. The novel role of preS2 would be useful to further unravel the mechanisms of imbalanced apoptosis during HBV infection and provides a potential therapeutic target for HBV-related diseases. |
| Keywords: | Hepatitis B virus PreS2 domain Apoptosis TRAIL |
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