A general method for the transfer of cloned genes to plant cells期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

A general method for the transfer of cloned genes to plant cells

Authors:	C H Shaw J Leemans C H Shaw M van Montagu J Schell

Affiliation:	1. Abteilung Schell, Max-Planck Institut für Züchtungsforschung, D5000 Köln 30 F.R.G. Tel. (0221) 50621;2. Department of Botany, University of Durham, DH1 3LE U.K. Tel. (0385) 64971, ext. 315;3. Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B1640 St-Genesius RodeBelgium Tel. (02) 6485540;4. Laboratorium voor Genetika, Rijksuniversiteit Gent, B9000 GentBelgium Tel. (91) 213492

Abstract:

Keywords:	Agrobacterium Ti-plasmid site-specific insertion rabbit β-globin axenic tobacco seedlings antibiotics, see Table II bp base pairs Denhardt's solution 0.02% Ficoll 400, 0.02% polyvinylpyrollidone, 0.02% bovine serum lbumin DMSO dimethyl sulphoxide EtBr ethidium bromide iV intermediate vector kb kilobase pairs MDal megadalton Occ octopine catabolism Pol I SDS sodium dodecyl sulphate T-DNA, Fraction of crown-gall DNA homologous to Ti-Plasmid T-region, segment of Ti-plasmid homologous to T-DNA Tn transposon [ ], denotes a plasmid-carrier state
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