Synthesis of long-chain fatty acids by microsomes of pigeon liver |
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| Affiliation: | 1. The Lipid Metabolism Laboratory, Veterans Administration Hospital, U.S.A.;2. The Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin U.S.A.;1. Institute of Environment and Sustainable Development, Banaras Hindu University, Varanasi, India;2. Navsari Agricultural University, Waghai, India;1. Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, PR China;2. Centre for Simulation and Modelling of Particulate Systems, Southeast University - Monash University Joint Research Institute, Suzhou 215123, PR China;3. Department of Chemical Engineering, Monash University, Clayton, Vic 3800, Australia;1. Solar Cell Applications Research Lab, Department of Physics, Government College University Lahore, Lahore 54000, Punjab, Pakistan;2. Department of Chemistry, Government College University Faisalabad, Pakpattan Road, Sahiwal 57000, Pakistan;3. Faculty of Pharmacy, The University of Lahore, Lahore 54000, Pakistan;4. Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, Punjab, Pakistan;5. Core Research Facilities, King Fahd University of Petroleum & Minerals, Dhahran 31261, Saudi Arabia;6. Departament d’Enginyeria Química, Universitat Rovira i Virgili, 43007 Tarragona, Spain;7. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;8. Department of Physics, Riphah Institute of Computing and Applied Sciences (RICAS), Riphah International University, 14 Ali Road, Lahore, Pakistan;1. Environmental Process Dynamics, Inc., 4117 Spinnaker Ln, Okemos, MI, USA;2. Gas Technology Institute, 1700 S. Mount Prospect Rd., Des Plaines, IL, USA;1. Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, India;2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India |
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| Abstract: | Results are presented which show that pigeon liver microsomes contain an enzyme system capable of synthesizing saturated and unsaturated C-18 and C-20 fatty acids by the addition of malonyl-CoA to shorter chain fatty acids. Since no malonyl-CoA was incorporated into the first five “two carbon” units of synthesized oleic acid (pelargonic acid), it is concluded that the shortest chain fatty acid elongated by this system is decanoic acid. This conclusion is supported by the relatively high specific radioactivity of the carboxyl group in stearic and oleic acids synthesized from 1,3-14C-malonyl-CoA and by the failure of octanoyl-CoA to stimulate the incorporation of malonyl-CoA into fatty acids. Requirements for ATP and a reduced nucleotide for microsomal fatty acid synthesis are also reported. The role of ATP in this system is discussed, and it is concluded that ATP probably functions in the activation of primer fatty acid units and in the synthesis of phospholipids. The latter are major end products of microsomal fatty acid synthesis. |
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