Variants of carnation (Dianthus caryophyllus L.) obtained by organogenesis from irradiated petals |
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Authors: | Marie-Hélène Simard Nicole Michaux-Ferriere André Silvy |
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Affiliation: | (1) CEN de Cadarache, DPVE, 13108 St-Paul-Les Durance;(2) Laboratoire BIOTROP, CIRAD, BP 5035, 34032 Montpellier Cédex |
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Abstract: | ![]() To obtain carnation variants differing from those produced by organogenesis alone, in vitro petal cultures were subjected to gamma irradiation. Histological analysis revealed the surface origin of buds and the different steps in meristem formation. A dose of 40 Gy administered on the fourth day of culture produced variants of horticultural interest in Niky . This period corresponded to dedifferentiation of cells that subsequently developed into bubs.Abbreviations BA benzyladenine - NAA naphthaleneacetic acid |
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Keywords: | mutagenesis petal culture regeneration somaclonal variation |
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