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Variants of carnation (Dianthus caryophyllus L.) obtained by organogenesis from irradiated petals
Authors:Marie-Hélène Simard  Nicole Michaux-Ferriere  André Silvy
Affiliation:(1) CEN de Cadarache, DPVE, 13108 St-Paul-Les Durance;(2) Laboratoire BIOTROP, CIRAD, BP 5035, 34032 Montpellier Cédex
Abstract:
To obtain carnation variants differing from those produced by organogenesis alone, in vitro petal cultures were subjected to gamma irradiation. Histological analysis revealed the surface origin of buds and the different steps in meristem formation. A dose of 40 Gy administered on the fourth day of culture produced variants of horticultural interest in lsquoNikyrsquo. This period corresponded to dedifferentiation of cells that subsequently developed into bubs.Abbreviations BA benzyladenine - NAA naphthaleneacetic acid
Keywords:mutagenesis  petal culture  regeneration  somaclonal variation
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