Identification of somaclonal variants of sugarcane (Saccharum spp.) resistant to sugarcane mosaic virus via RAPD markers |
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Authors: | Maira Oropeza Palmira Guevara Eva de García José Luis Ramírez |
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Institution: | (1) Centro de Botánica Tropical and Grupo de Genética Molecular, Facultad de Ciencias, Universidad Central de Venezuela, Apdo 47525 Caracas, 1041-A, Venezuela |
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Abstract: | Somaclonal variants resistant to sugarcane mosaic virus (SCMV) were obtained from susceptible sugarcane cv PR62258 through
somatic embryogenesis by increasing the number of subcultures of the embryogenic callus tissue in MS medium with 3 mg/L 2,4-dichlorophenoxyacetic
acid. Transfers were made at 30-day intervals for 1, 2 or 3 subcultures. Two somaclones, namely AT626 and BT627, were selected
by their resistance to SCMV. These subclones have maintained the resistance trait over seven years of testing in the field.
In this report we identified the somaclonal SCMV resistant variants from the maternal line and the nonresistant somaclones,
using the RAPD technique. |
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Keywords: | Saccharum spp somaclonal variation sugarcane mosaic virus RAPD |
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