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Analytical Guidelines for co-fractionation Mass Spectrometry Obtained through Global Profiling of Gold Standard Saccharomyces cerevisiae Protein Complexes
Affiliation:1. Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia;2. Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia;3. School of Chemistry, University of New South Wales, Sydney, New South Wales, Australia;4. School of Mathematics and Statistics, University of New South Wales, Sydney, New South Wales, Australia;5. Stanley Institute for Cognitive Genomics, Cold Spring Harbor Laboratory, Woodbury, New York, USA;6. Department of Molecular Sciences, Macquarie University, Sydney, New South Wales, Australia
Abstract:
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  • Highlights
    • •Guidelines for studying protein complexes via co-fractionation mass spectrometry.
    • •A novel procedure for profiling gold standard protein complexes in CF-MS data.
    • •Recommendations for efficient CF-MS fractionation collection.
    • •Scoring metric recommendations for precise and sensitive CF-MS data analysis.
    Keywords:co-fractionation mass spectrometry  protein correlation profiling  protein complexes  protein-protein interactions  Saccharomyces cerevisiae  Protein complex analysis  Yeast*  Bioinformatics  Chromatography
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