Benefits of Collisional Cross Section Assisted Precursor Selection (caps-PASEF) for Cross-linking Mass Spectrometry期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Benefits of Collisional Cross Section Assisted Precursor Selection (caps-PASEF) for Cross-linking Mass Spectrometry

Authors:	Barbara Steigenberger Henk WP van den Toorn Emiel Bijl Jean-Franois Greisch Oliver Rther Markus Lubeck Roland J Pieters Albert JR Heck Richard A Scheltema

Institution:	1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands;2. Netherlands Proteomics Centre, Utrecht, The Netherlands;3. Bruker Daltonik GmbH, Bremen, Germany;4. Department of Chemical Biology & Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands

Abstract:	Download : Download high-res image (129KB) Download : Download full-size image Highlights •Cross-linked peptides are physically separated from mono-linked peptides in the gas-phase by TIMS ion mobility. •Development of a novel data acquisition routine that a-priori distinguishes cross-linked from mono-linked peptides called caps-PASEF. •First application of PhoX-driven cross-linking mass spectrometry on the timsTOF Pro. •Application of cross-linking mass spectrometry to medium to high complexity samples.

Keywords:	Mass spectrometry protein cross-linking* crosslinking data evaluation macromolecular complex analysis separation technologies ion mobility
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