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Kir2.1 Interactome Mapping Uncovers PKP4 as a Modulator of the Kir2.1-Regulated Inward Rectifier Potassium Currents
Authors:Sung-Soo Park  Daniela Ponce-Balbuena  Rork Kuick  Guadalupe Guerrero-Serna  Justin Yoon  Dattatreya Mellacheruvu  Kevin P Conlon  Venkatesha Basrur  Alexey I Nesvizhskii  Jos Jalife  Jean-Franois Rual
Institution:1. Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, USA;2. Department of Internal Medicine and Center for Arrhythmia Research, University of Michigan, Ann Arbor, Michigan, USA;3. Department of Biostatistics, School of Public Health, University of Michigan, Ann Arbor, Michigan, USA;4. Department of Computational Medicine & Bioinformatics, University of Michigan, Ann Arbor, Michigan, USA;5. Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain;6. Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares, Madrid, Spain
Abstract:
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  • Highlights
    • •Generation using BioID of a map of the Kir2.1 interactome with 218 interactions.
    • •Identification of Kir2.1WT- versus Kir2.1Δ314-315-preferred interactors.
    • •Identification of the desmosome protein PKP4 as a new modulator of IKir2.1 currents.
    Keywords:Protein-protein interactions*  cardiovascular disease  cardiovascular function or biology  mass spectrometry  macromolecular complex analysis  BioID  cardiomyopathy  inward rectifier potassium current  Kir2  1  PKP4
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