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Mitochondrial Oxidative Phosphorylation Complex Regulates NLRP3 Inflammasome Activation and Predicts Patient Survival in Nasopharyngeal Carcinoma
Institution:3. Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan;4. Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan;5. Department of Medicine, Mackay Medical College, New Taipei City 252, Taiwan;6. Department of Radiation Oncology, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan;12. Department of Pathology, Chang Gung Memorial Hospital, Linkou, and Chang Gung University College of Medicine, Taoyuan, Taiwan;8. Department of Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan;9. Department of Biomedical Sciences, University of the Pacific, San Francisco, California 94103;10. Center for Molecular and Clinical Immunology, Chang Gung University, Taoyuan, Taiwan;123. Chang Gung Immunology Consortium, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan;84. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan;95. Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan City 333, Taiwan;106. Department of Otolaryngology - Head & Neck Surgery, Chang Gung Memorial Hospital, Linkou, Taoyuan, Taiwan
Abstract:
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  • Highlights
    • ?Flow cytometry analysis is used to isolate ASC speck(+) NPC cells.
    • ?Proteome analysis of ASC speck(+) NPC cells reveals enriched mitochondrial OxPhos proteins.
    • ?OxPhos proteins mediate NLRP3 inflammasome activation through mtROS.
    • ?OxPhos proteins, NDUFB8 and ATP5B are correlated with NPC local recurrence.
    Keywords:iTRAQ  inflammatory response  mitochondria function or biology  oxidative stress  cancer biology  nasopharyngeal carcinoma
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