Intracardiac injection of matrigel induces stem cell recruitment and improves cardiac functions in a rat myocardial infarction model |
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| Authors: | Lailiang Ou Wenzhong Li Yue Zhang Weiwei Wang Jun Liu Heiko Sorg Dario Furlani Ralf Gäbel Peter Mark Christian Klopsch Liang Wang Karola Lützow Andreas Lendlein Klaus Wagner Doris Klee Andreas Liebold Ren‐Ke Li Deling Kong Gustav Steinhoff Nan Ma |
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| Institution: | 1. Reference‐ and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, Rostock, Germany;2. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, China;3. Institute of Polymer Research, GKSS Research Center, Geesthacht, Germany;4. Department of Anesthesia, Klinikum Südstadt Rostock, Rostock, Germany;5. Institute of Technical and Macromolecular Chemistry Aachen, Aachen, Germany;6. Division of Cardiovasculary Surgery, Toronto General Hospital and the University of Toronto, Toronto, Canada |
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| Abstract: | Matrigel promotes angiogenesis in the myocardium from ischemic injury and prevents remodelling of the left ventricle. We assessed the therapeutic efficacy of intracardiac matrigel injection and matrigel‐mediated stem cell homing in a rat myocardial infarction (MI) model. Following MI, matrigel (250 μl) or phosphate‐buffered solution (PBS) was delivered by intracardiac injection. Compared to the MI control group (MI‐PBS), matrigel significantly improved left ventricular function (n= 11, P < 0.05) assessed by pressure–volume loops after 4 weeks. There is no significant difference in infarct size between MI‐matrigel (MI‐M; 21.48 ± 1.49%, n= 10) and MI‐PBS hearts (20.98 ± 1.25%, n= 10). The infarct wall thickness of left ventricle is significantly higher (P < 0.01) in MI‐M (0.72 ± 0.02 mm, n= 10) compared with MI‐PBS (0.62 ± 0.02 mm, n= 10). MI‐M hearts exhibited higher capillary density (border 130.8 ± 4.7 versus 115.4 ± 6.0, P < 0.05; vessels per high‐power field HPF; 400×], n= 6) than MI‐PBS hearts. c‐Kit+ stem cells (38.3 ± 5.3 versus 25.7 ± 1.5 c‐Kit+ cells per HPF 630×], n= 5, P < 0.05) and CD34+ cells (13.0 ± 1.51 versus 5.6 ± 0.68 CD34+ cells per HPF 630×], n= 5, P < 0.01) were significantly more numerous in MI‐M than in MI‐PBS in the infarcted hearts (n= 5, P < 0.05). Intracardiac matrigel injection restores myocardial functions following MI, which may attribute to the improved recruitment of CD34+ and c‐Kit+ stem cells. |
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| Keywords: | cardiac regeneration ischemia matrigel stem and progenitor cells |
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