Responses of embryogenic mango cultures and seedling bioassays to a partially purified phytotoxin produced by a mango leaf isolate of Colletotrichum gloeosporioides penz |
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Authors: | S Jayasankar Richard E Litz Dennis J Gray Pamela A Moon |
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Institution: | (1) Tropical Research and Education Center, University of Florida, 18905 SW 280 Street, 33031-3314 Homestead, Florida;(2) Central Florida Research and Education Center, University of Florida, 5336 University Avenue, 34748-8232 Leesburg, Florida |
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Abstract: | Summary
Colletotrichum gloeosporioides Penz., the causal agent of mango anthracnose, produces a phytotoxin in vitro. The partially purified phytotoxin, presumably
colletotrichin, caused anthracnose-like symptoms on young mango leaves, was toxic to embryogenic suspension cultures of two
mango cultivars, ‘Hindi’ and ‘Carabao,’ and inhibited in vitro seed germination of two nonhosts, lettuce and tobacco. There
were linear relationships between concentration of the partially purified phytotoxin and mortality of mango embryogenic cultures.
Embryogenic cultures grown in the presence of the partially purified phytotoxin showed significantly lower growth rates than
the controls. Similarly, embryogenic cultures grown in the presence of 40% (vol/vol) fungal culture filtrate showed significantly
lower growth rates than unchallenged controls. Medium containing 40% (vol/vol) Czapek-Dox fungal broth did not reduce growth
of embryogenic cultures, indicating the production of phytotoxin in vitro. The results suggest that either fungal culture
filtrate or purified phytotoxin can be used as in vitro selection agents to screen for resistance to this fungus. |
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Keywords: | mango anthracnose somatic embryogenesis phytotoxin Colletotrichum gloeosporioides |
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