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POPU金钱豹mtDNA ND5基因序列及其遗传变异
引用本文:朱军,张阳,马恩波,任竹梅. POPU金钱豹mtDNA ND5基因序列及其遗传变异[J]. 动物分类学报, 2012, 37(2): 263-268
作者姓名:朱军  张阳  马恩波  任竹梅
作者单位:朱军 (山西省生物多样性研究中心 太原 030012) ; 张阳 (山西大学生命科学学院 太原 030006) ; 马恩波 (山西大学应用生物学研究所 太原 030006) ; 任竹梅 (山西大学生命科学学院 太原 030006)
基金项目:国家自然科学基金,国家林业局基金项目资助
摘    要:
测定山西7个不同地域金钱豹9个体mtDNA ND5基因部分序列,结合GenBank下载的37条序列,进行遗传多样性分析,用豹属的狮Panthera leo和虎Panthera tigris作外群,构建其不同单倍型的NJ分子聚类关系。46条序列共产生18个单倍型,单倍型多样度和核苷酸多样度指数表明金钱豹的遗传多样性比较丰富,AMOVA分析显示其种群间出现显著的遗传分化。单倍型聚类分析表明,亚洲和非洲种群尽管有一定的分化,但其置信度很低,不足以形成各自独立的分支。

关 键 词:金钱豹  mtDNA  ND5  基因序列  遗传多样性

POPULATION GENETIC DIVERSITY OF PANTHERA PARDUS BASED ON MTDNA ND5 GENE SEQUENCES
ZHU Jun,ZHANG Yang,MA En-Bo,REN Zhu-Mei. POPULATION GENETIC DIVERSITY OF PANTHERA PARDUS BASED ON MTDNA ND5 GENE SEQUENCES[J]. Acta Zootaxonomica Sinica, 2012, 37(2): 263-268
Authors:ZHU Jun  ZHANG Yang  MA En-Bo  REN Zhu-Mei
Affiliation:1. Biodiversity Research Central in Shanxi, Taiyuan 030002, China 2. School of Life Science, Shanxi University, Taiyuan 030006, China 3. Institute of Applied Biology, Shanxi University, Taiyuan 030006, China
Abstract:
The mtDNA ND5 genes (316 bp) were sequenced from 9 individuals of Panthera pardus from Shanxi, China, combined with 37 sequences from GenBank, and two outgroup species (Panthera leo and Panthera tigris). Eighteen haplotypes from 46 individuals of mtDNA ND5 gene of P. pardus were examined. The haplotype diversity and nucleotide diversity indicated that the genetic diversity of P. pardus is very high. Analysis of molecular variance (AMOVA) revealed that significant differentiation among the P. pardus populations. However, the phylogenetic tree showed that the P. pardu population from African and Asia form two groups but with low boot-strap value.
Keywords:Panthera pardus  mtDNA  ND5 gene sequences  genetic diversity.
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