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Isolation and characterization of 12 novel DNA microsatellites in the western rainbowfish, Melanotaenia australis
Authors:Young Michael J  Simmons Leigh W  Evans Jonathan P
Affiliation:Centre for Evolutionary Biology, School of Animal Biology, University of Western Australia, Crawley, WA 6009, Australia.
Abstract:
Microsatellites were isolated from the western rainbowfish, Melanotaenia australis, for use as molecular markers. Twelve polymorphic loci were found. When characterized using 32 individuals, these had between two and 24 alleles each, and observed heterozygosities ranged from 0.00 to 0.97. Ten loci were in Hardy-Weinberg equilibrium, and all of them showed independent inheritance. These loci will be useful for the study of molecular subdivision in this species.
Keywords:FIASCO protocol  Melanotaenia splendida australis  Melanotaeniidae  population genetics
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