A multiplex PCR assay to distinguish among three sympatric marsupial taxa from Huon Peninsula, Papua New Guinea, using the mitochondrial control region |
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Authors: | McGreevy T J Dabek L Husband T P |
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Affiliation: | Department of Natural Resources Science, Coastal Institute, University of Rhode Island, Kingston, RI 02881, USA Tree Kangaroo Conservation Program, Department of Conservation, Woodland Park Zoo, Seattle, WA 98103, USA. |
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Abstract: | ![]() Matschie’s tree kangaroo (Dendrolagus matschiei) is an endangered arboreal macropodid marsupial that is difficult to locate in the wild. The Tree Kangaroo Conservation Program noninvasively collected D. matschiei faecal samples, but a high percentage of samples were misidentified and belonged to two terrestrial macropods, New Guinea pademelon (Thylogale browni) and small dorcopsis (Dorcopsulus vanheurni). We developed species-specific primers that produced a unique sized mitochondrial DNA control region gene product for each taxon and achieved an 89% correct assignment rate. The species-specific primers can increase the accuracy of D. matschiei density estimates and facilitate additional genetic analyses of wild D. matschiei. |
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Keywords: | endangered species Matschie’s tree kangaroo (Dendrolagus matschiei) noninvasive sampling New Guinea pademelon (Thylogale browni) small dorcopsis (Dorcopsulus vanheurni) species identification |
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