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A multiplex PCR assay to distinguish among three sympatric marsupial taxa from Huon Peninsula, Papua New Guinea, using the mitochondrial control region
Authors:McGreevy T J  Dabek L  Husband T P
Affiliation:Department of Natural Resources Science, Coastal Institute, University of Rhode Island, Kingston, RI 02881, USA Tree Kangaroo Conservation Program, Department of Conservation, Woodland Park Zoo, Seattle, WA 98103, USA.
Abstract:
Matschie’s tree kangaroo (Dendrolagus matschiei) is an endangered arboreal macropodid marsupial that is difficult to locate in the wild. The Tree Kangaroo Conservation Program noninvasively collected D. matschiei faecal samples, but a high percentage of samples were misidentified and belonged to two terrestrial macropods, New Guinea pademelon (Thylogale browni) and small dorcopsis (Dorcopsulus vanheurni). We developed species-specific primers that produced a unique sized mitochondrial DNA control region gene product for each taxon and achieved an 89% correct assignment rate. The species-specific primers can increase the accuracy of D. matschiei density estimates and facilitate additional genetic analyses of wild D. matschiei.
Keywords:endangered species  Matschie’s tree kangaroo (Dendrolagus matschiei)  noninvasive sampling  New Guinea pademelon (Thylogale browni)  small dorcopsis (Dorcopsulus vanheurni)  species identification
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