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New sequence-tagged site molecular markers for identification of sex in Distichlis spicata
Authors:Eppley Sarah M  O'Quinn Robin  Brown Anna L
Affiliation:Department of Biology, Portland State University, PO Box 751, Portland, OR 97207-0751, USA.
Abstract:
Sex-linked molecular markers have become valuable tools for understanding sex ratio evolution and sex-specific physiology in pre-reproductive plants. To develop new accurate methods for sexing Distichlis spicata juveniles and nonflowering individuals, we converted a random amplified polymorphic DNA-polymerase chain reaction marker that co-segregated with the female phenotype into a set of sequence-tagged site markers. We tested the marker pair on known males and females from populations in Oregon and California. A single band was obtained for all female samples but never for males.
Keywords:dioecious  PCR  RAPD  sequence‐tagged site  sex
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