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Isolation and characterization of nine polymorphic microsatellite loci in the endangered shrub Disanthus cercidifolius var. longipes (Hamamelidaceae)
Authors:Gao Puxin  Yang Aihong  Yao Xiaohong  Huang Hongwen
Affiliation:Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan, Hubei 430074, China, Lushan Botanical Garden, Jiangxi Province and the Chinese Academy of Sciences, Lushan, Jiangxi 332900, China, Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
Abstract:Nine polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Disanthus cercidifolius var. longipes. Microsatellite polymorphism was investigated using 24 individuals from one natural population. The observed number of alleles per locus ranged from two to four. Observed and expected heterozygosities ranged from 0.17 to 0.92 and from 0.16 to 0.72, respectively. These polymorphic microsatellite loci provide useful tools for the ongoing population genetic studies of D. cercidifolius var. longipes.
Keywords:conservation genetics  Disanthus cercidifolius var. longipes  Hamamelidaceae  microsatellite
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