臭鼩血清蛋白和六种组织乳酸脱氢酶同工酶的研究

本实验对臭鼩的血清蛋白及心肌、骨骼肌、肾脏、脾脏、肝脏,睾丸6种组织器官的乳酸脱氢酶(LDH)同工酶进行了聚丙烯酰胺凝胶盘状电泳的分析研究。臭鼩血清蛋白存在15—17条带,各组织的LDH同工酶均由5条带构成,其中心肌LDH-1、LDH-2和肾脏LDH-1各出现1条亚带。

STUDIES ON THE SERUM AND LACTATE DEHYDROGENASE ISOZYMES OF TISSUES FROM SUNCUS MURINUS

The present paper reports the analysis of serum protein and lactate dehydrogenase (LDH) isozymes in six kinds of tissues of Suncus murinus murinus. The method used was the polyacrylamide gel electrophoresis. The serum protein and LDH isozymes in tissues of heart, muscle, kidney, spleen, liver and testis both have specificity. There were 15-17 bands in the serum. 5 LDH bands could be found in all kinds of tissues. Different bands are referred to as LDH-1, LDH-2, LDH-3, LDH-4 and LDH-5 in order of relative electrophoretic mobility from cathode towards anode. The mobility of the LDH isozymes have been found obviously slow and the LDH-5 bands have got close to the cathode. In the ratios of A subunit and B subunit, A subunits have had the preponderance in tissues of muscle, spleen and liver while B subunit in tissue of heart and both A subunits and B subunits have not got dominant position in tissues of kidney and testis. In the LDH-1, LDH-2 of heart and LDH-1 of kidney sub-bands could be detected