Tracing carbon monoxide uptake by Clostridium ljungdahlii during ethanol fermentation using 13C-enrichment technique |
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Authors: | Seok-In Yun Seong-Joo Gang Hee-Myong Ro Min-Jin Lee Woo-Jung Choi Seong-Gu Hong Kwon-Kyoo Kang |
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Institution: | 1. Department of Bio Environmental Chemistry and Institute of Life Science and Natural Resources, Wonkwang University, Iksan, 570-749, Republic of Korea 2. Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea 3. Department of Rural and Bio-Systems Engineering, Chonnam National University, Gwangju, 500-757, Republic of Korea 4. Department of Bioresource and Rural Systems Engineering, Hankyong National University, Ansung, 456-749, Republic of Korea 5. Department of Horticulture, Hankyong National University, Ansung, 456-749, Republic of Korea
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Abstract: | Conversion of synthesis gas (CO and H2) to ethanol can be an alternative, promising technology to produce biofuels from renewable biomass. To distinguish microbial utilization of carbon source between fructose and synthesis gas CO and to evaluate biological production of ethanol from CO, we adopted the 13C-enrichment of the CO substrate and hypothesized that the residual increase in δ13C of the cell biomass would reflect the increased contribution of 13C-enriched CO. Addition of synthesis gas to live culture medium for ethanol fermentation by Clostridum ljungdahlii increased the microbial growth and ethanol production. Despite the high 13C-enrichment in CO (99 atom % 13C), however, microbial δ13C increased relatively small compared to the microbial growth. The uptake efficiency of CO estimated using the isotope mass balance equation was also very low: 0.0014 % for the low CO and 0.0016 % for the high CO treatment. Furthermore, the fast production of ethanol in the early stage indicated that the presence of sugar in fermentation medium would limit the utilization of CO as a carbon source by C. ljungdahlii. |
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