Ectomycorrhiza formation ofTricholoma matsutake isolates on seedlings ofPinus densiflora in vitro |
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Authors: | Akiyoshi Yamada Ken Maeda Masatake Ohmasa |
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Institution: | (1) Ibaraki Prefectural Forestry Center, Naka-machi, Naka-gun, 311-0122 Ibaraki, Japan;(2) Present address: Faculty of Agriculture, Shinshu University, Minami-minowa-mura, Kamiina-gun, 399-4598 Nagano, Japan |
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Abstract: | Tricholoma matsutake forms ectomycorrhizas withPinus densiflora under field conditions. The present study aimed to test the ability ofT. matsutake isolates to form mycorrhizas with aseptic seedlings ofP. densiflora in vitro. Pine seeds were germinated aseptically on a nutrient agar medium, and pairs of 1-wk-old seedlings were transplanted
into polymethylpentene bottles containing autoclaved sphagnum moss/vermiculite substrate. The substrate was saturated with
nutrient medium containing glucose. At the same time, the bottles were inoculated with aT. matsutake isolate. Three mo after inoculation, the fungus formed a sheath and Hartig net on the pine lateral roots. Ectomycorrhizas
were also confirmed on 4-6-mo-old seedlings which showed the same or slinghtly better growth than the control plants. These
results indicate that culturedT. matsutake mycelium can form true ectomycorrhizas withP. densiflora seedlings in vitro. |
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Keywords: | aseptic culture edible mushroom Hartig net in vitro ectomycorrhiza symbiosis |
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