萝卜溶菌酶诱导条件的研究

分别以不同浓度（Ａ_（４５０ｎｍ）表示）的高压灭活白色葡萄球菌、大肠杆菌及水杨酸对萝卜溶菌酶进行不同时间的诱导．结果表明，诱导时间２４ｈ效果最好，白色葡萄球菌最适浓度Ａ_（４５０ｎｍ）为０．９４，酶的活力提高了２．３４倍；大肠杆菌最适浓度是Ａ_（４５０）ｎｍ为０．５５，诱导酶活力提高了０．７５倍，以０．２％的水杨酸为诱导物时，第４ｄ和第８ｄ的效果最好，酶活力分别提高了２．４和１．７倍．以酶作用产物进行田间叶面喷施和叶面喷施加根部灌施进行诱导，４８ｈ后酶活力提高２．１６和３．６６倍．

Investigation of Condition of Induction on Lysozyme from Raphanus sativus leaves

A lysozyme from Raphanus sativus leaves in vitro was induced by different concentration of salicylic acid,S. ablus and E. coli killed with high temperature. Analysis showed that when A_(450nm) of killed S. ablus was 0. 94 and induction time was 24h,the effct was the best,the specific activity of the lysozyme was increased by 2.34-fold; the optimum concentration (A_(450)) of E.coli was 0. 55,the specific activity was increased by 0. 57-fold,the optimum induction time of salicylic acid was the fourth day and the eighth day,the specific activity was incressed by 2. 4-fold and 1.7-fold respectively.