The characteristics of purified HL60 tuftsin receptors

Summary The purification and characteristics of purified HL60 tuftsin receptors are described. Purification was accomplished by affinity chromatography similar to that described earlier, wherein a tuftsin analog Thr-LysPro-Pro-Arg, is covalently linked at the N group to a solid support. The receptor consists presumably of two subunits approximately 66 KDa and 57 KDa. The dissociation constant of the receptor complex is 4.7 × 10^–8 M with 5 × 104 receptors per cell. It can form oligomers with an Mr of about 560 KDa suggesting an octomeric structure, assuming the same number of each subunit is associated.Abbreviations CHAPS 3-(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate - DCC Dicyclohexylcarbodiimide - DMSO Dimethylsulfoxide - DSS Disuccinimidylsuberate - FCS Fetal Calf Serum - HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid - MMP Macrophage/Monocyte and Polymorphonuclear granulocytes - Mr Relative Molecular mass - NaDodSO₄ Sodium Dodecyl Sulfate - PEG Polyethylene Glycol - SP Sucrose Phosphate buffer - TFMS Trifluoromethanesulfonic Acid